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Uji sensivitas dan spesifisitas antibodi anti fim-C Salmonella typhi sebagai dasar pengembangan deteksi demam tifoid

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Demam tifoid adalah penyakit yang diderita oleh banyak orang di negara berkembang termasuk Indonesia. Hingga saat ini metode diagnosis demam tifoid dengan spesifisitas dan sensitivitas tinggi masih terus dikembangkan. Penelitian ini bertujuan untuk memperoleh informasi tentang sensitivitas dan spesifisitas antibodi anti-Fim-C Salmonella typhi terhadap protein Fim-C, ekstrak protein bakteri S.typhi dan ekstrak protein bakteri lainnya. Metode yang digunakan dalam penelitian ini adalah metode ELISA (Enzyme Linked Immunosorbent Assay) dan Western Immunoblotting. Antigen yang digunakan adalah Recombinant Protein Fim-C S.typhi penelitian sebelumnya. Hasil studi ELISA menunjukkan antibodi anti-Fim-C sensitif terhadap antibodi Fim-C rekombinan, dalam mendeteksi antigen terendah dengan konsentrasi 12,5 ng. Hasil metode Western Blot menunjukkan bahwa antibodi anti Fim-C secara spesifik mengidentifikasi antigen protein rekombinan Fim-C dalam pita protein ± 31 kDa, ekstrak protein bakteri S.typhi yang berukuran ± 29 dan ± 60 kDa, ekstrak protein bakteri S.typhimurium. ± 28 dan ± 60 kDa, ekstrak protein bakteri E.coli berukuran ± 34 kDa dan ± 60 kDa dan ekstrak protein bakteri Shigella berukuran ± 60 kDa. Berdasarkan hasil penelitian dapat disimpulkan bahwa antibodi anti Fim-C S.typhi berpotensi dikembangkan sebagai dasar pengembangan alat pendeteksi demam tifoid.

Typhoid fever is a disease that is suffered by many people in developing countries including Indonesia. Until now the method of diagnostic typhoid fever with high specificity and sensitivity is still being developed. This study aims to obtain information on the sensitivity and specificity of anti-Fim-C antibodies Salmonella typhi to Fim-C protein, S.typhi bacteria protein extract and other bacterial protein extracts. The method used in this research is the method of ELISA (Enzyme Linked Immunosorbent Assay) and Western Immunoblotting. Antigen used is Recombinant Protein Fim-C S.typhi previous research. The results of the ELISA study showed anti-Fim-C antibodies were sensitive to recombinant Fim-C antibody, in detecting the lowest antigen with a concentration of 12.5 ng. The results of the Western Blot method showed that anti Fim-C antibodies specifically identified Fim-C recombinant protein antigens in ± 31 kDa protein bands, S.typhi bacteria protein extracts measuring ± 29 and ± 60 kDa, bacterial protein extracts S.typhimurium measuring ± 28 and ± 60 kDa, E.coli bacteria protein extract measuring ± 34 kDa and ± 60 kDa and Shigella bacteria protein extract measuring ± 60 kDa. Based on the results can be concluded that antibody anti Fim-C S.typhi can potentially be developed as the basis for development of tifoid fever detection tool.

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Ketersediaan

Informasi Detail

Judul Seri

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No. Panggil

SK 16141

Penerbit

Jakarta : Prodi Kimia FMIPA UNJ., 2018

Deskripsi Fisik

viii, 60 lembar : il. ; 30 cm.

Bahasa

Indonesia

ISBN/ISSN

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Klasifikasi

NONE

Tipe Isi

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Tipe Media

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Tipe Pembawa

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Edisi

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Subjek

Demam Tifoid

Info Detail Spesifik

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Pernyataan Tanggungjawab

Anis Marsela

Versi lain/terkait

Lampiran Berkas

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